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. Author manuscript; available in PMC: 2022 May 4.
Published in final edited form as: J Vis Exp. 2019 Apr 23;(146):10.3791/59544. doi: 10.3791/59544

Figure 3: EHBDOs express markers of progenitor and mature biliary cells.

Figure 3:

(A-C). EHBDOs were analyzed by immunofluorescence staining for markers epithelial (A, B. E-cadherin, red), progenitor (A. PDX1, green), and differentiated (B. CK19, green; and C. a-AT, red) biliary cells. Scale bars = 25 μm. *, lumen. (D). EHBDOs were analyzed for abundance of Pdx1, Ck19, Sox9, Aqp1, and Cftr mRNA by qRT-PCR (mean +/− SEM relative to expression of Hprt).