Figure EV4. Modeling Aci2’s lags as dependent on its population density at the onset of its diauxic shift (instead of initial alanine supply) prevents Pa from excluding Aci2.

1. Using the lag times presented in Fig 2H and the population sizes at the onset of Aci2’s diauxic shift presented in Fig 2C allows Aci2’s lag time to be alternatively expressed as a function of its population size at the onset of its diauxic shift. Circles represent lag time data and the line defines the piecewise linear function used for modeling. Whether Aci2’s lag is most accurately modeled as a function of alanine supply or of population size at the time of its diauxic shift could not be definitively determined (Appendix Fig S8).
2. Steady‐state phase space for the Aci2 versus Pa on alanine and glutamate competition at various resource supply ratios and dilution factors when modeling Aci2’s lag as a function of its population size at the onset of the diauxic shift. The most significant change relative to the original prediction (Fig 5B) is that Pa never fully excludes Aci2. Pa cannot exclude Aci2 because as Aci2 is driven extinct its population size at its diauxic shift as well as its diauxic lag time converge to zero, eliminating the period when Pa can catch up to Aci2.