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. 2022 May 4;17(5):e0266980. doi: 10.1371/journal.pone.0266980

Fig 3. AST enzymatic activity in BOXR1030 CAR T cells.

Fig 3

(A) Schematic describing the role of GOT2 in mitochondrial conversion of glutamate to aKG and OAA to aspartate. Image created with biorender.com. (B) Intracellular aspartate levels were measured using a plate-based colorimetric assay comparing BOXR1030 and control CAR T cells under non-stimulated conditions. (C) BOXR1030 and control CAR T cells were activated for 8 hours with GPC3+ Hep3B target cells and evaluated for AST activity. Data represented as mean +/- SD of two technical replicates.