Fig 4. Place learning, cohort A.

(A) Example of correct (green) and incorrect (yellow) corner layout for a rat assigned to corner 4. (B) Day factor, (C) Sex factor and (D) Run factor comparisons of area under the curve (AUC) for correct visit learning curves of individual animals by sex, program day, and Run. (E) Learning curves showing the fractional accumulation (y-axis) of correct visits over drinking session time (x-axis), reset every 180 minutes, by sex and Run. (F) For Run-1, 12 male and 12 female rats were tested. All 24 rats were included in the analysis of Run-1. For Run-2, 12 male and 10 female rats were tested, since two females died during the time between Runs. During Run-2, two males were excluded from the analysis of day 1 and 2, and one male was excluded from the analysis of day 3. These animals were excluded because they visited corners less than 25 times during the drinking session. All data represented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). See Tables 4 and 5 for statistical analysis. Males (M or ♂) are colored as red (Run-1) and light red (Run-2). Females (F or ♀) are colored as blue (Run-1) or light blue (Run-2).