Skip to main content
. 2022 May 4;20(5):e3001616. doi: 10.1371/journal.pbio.3001616

Fig 2. Field isoforms of PfMDR1 differ significantly in their capacities for antimalarial drug transport.

Fig 2

(a–i) Field isoforms of PfMDR1 transported the antimalarial drugs [3H]lumefantrine (a), [3H]mefloquine (b), [3H]chloroquine (c), [3H]quinidine (d), [3H]amodiaquine (e), [3H]piperaquine (f), [3H]dihydroartemisinin (g), methylene blue (h), and quinacrine (i). (j–l) All of the field isoforms of PfMDR1 also transported the human P-gp substrates rhodamine B (j) and [3H]vinblastine (k), but none transported [3H]amantadine (l). The transport of methylene blue, quinacrine, and rhodamine B was detected using the intrinsic fluorescence of these compounds and a fluorescence-based transport assay (see S2 Text). The data are the mean of n = 4 independent experiments (each yielding similar results and overlaid as individual data points), and the error is the SEM. The asterisks denote a significant difference from PfMDR1NYSND; *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant (1-way ANOVA). The data underlying this figure is supplied in S3 Data. ne, nonexpressing oocytes; PfMDR1, Plasmodium falciparum multidrug resistance protein 1.