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. 2022 May 4;8(18):eabn8281. doi: 10.1126/sciadv.abn8281

Fig. 3. Differential expression of RAP2.12 driven by pSic-RAP2.12 or pTib-RAP2.12 results in contrasting opposite tolerance to flooding and drought.

Fig. 3.

(A) The relative expression level of RAP2.12 in shoots after air or submergence treatments (20 hours) in Sichuan and Tibet accessions. (B) Phenotypic and survival comparison between Tibet and a Tibet NIL containing introgressed RAP2.12 (Sichuan), NILRAP2.12-Sic. Phenotypes after 48 hours of dark/submergence and 7 days recovery. Scale bar, 2 cm. (C) The differential relative expression level of RAP2.12 in shoots between Sichuan and Tibet accessions in response to drought. (D) Comparison of drought tolerance in Col-0, pSic-RAP2.12:RAP2.12-GUS, and pTib-RAP2.12:RAP2.12-GUS (all in Col-0 background). Phenotypes after 14 days without watering, followed by 2-day rewatering. Scale bar, 2 cm. Data are means ± SE (n = 3 repeats). Different letters denote significant differences according to Tukey’s post hoc test (P < 0.05). **P < 0.01 (Student’s t test).