Skip to main content
. 2022 May 4;8(18):eabn8281. doi: 10.1126/sciadv.abn8281

Fig. 4. Occurrence of W box and WT box in the proximal promoter of RAP2.12 in the Brassicaceae and differential association with drought and aridity index.

Fig. 4.

(A) Representative leaf images of RAP2.12-GUS reporter lines in pSic-RAP2.12:GUS, pTib-RAP2.12:GUS, and piecewise substitutions of RAP2.12 promoter from Sichuan to Tibet accession (see also Fig. 2B) in 14-day-old seedlings after spraying with 30 μM ABA for 6 hours. Scale bars, 1 mm. (B) Haplotype network of the RAP2.12 promoter in A. thaliana over the world. Each haplotype is represented by a circle. The accessions with WT box and W box are divided into Hap-WT box and Hap-W box clades. The black dots indicate missing haplotype, and circle sizes represent relative frequency. (C) Geographic distribution of Hap-WT box and Hap-W box RAP2.12 on aridity index in haplotype network testing (data S1). (D) Phylogenetic analysis of RAP2.12 promoter in Brassicaceae species by the neighbor-joining method. The gene names or numbers and species names are given in the phylogenetic tree. The distributions of WT box and W box on the RAP2.12 orthologous gene promoter are displayed on the right, and the details of the sequence are shown in fig. S19.