Table 2. Digest buffer.

Component	Stock solution concentration	Amount (ml)	Final concentration
Triton X-100	10% (w/v)	5	0.5% (w/v)
EDTA	0.5 M (pH 8.0)	0.2	1 mM
Tris-HCI	1 M (pH 8.0)	5	50 mM
Sodium chloride (NaCl)	5 M	20	1 M
Proteinase K*	800 U/ml	1	8 U/ml
Water		68.8
Total		100

*Proteinase K was freshly added to the digest buffer.