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. 2022 May 4;8(18):eabn2748. doi: 10.1126/sciadv.abn2748

Fig. 4. dCas9-KRAB plus sgRNAs decreases eRNA expression and increases NELF binding at Arc promoter in the amygdala of control rats.

Fig. 4.

(A) dCas9-KRAB plus sgRNAs causes decreased Arc eRNA expression [Arc eRNA (−): Mann-Whitney U = 3.0, P = 0.015; eRNA (+): t10 = 2.715, P = 0.022, n = 6 in each group]. (B) dCas9-KRAB plus sgRNAs increases NELF binding at the Arc promoter (t10 = 2.487, P = 0.032, n = 6 in each group). (C) dCas9-KRAB plus sgRNAs produces no change in H3K27me3 occupancy at several sites of Arc (promoter −441 bp; Arc TSS, Arc distal region −2868 bp, n = 6 in each group) gene. (D) 3C assay shows that dCas9-KRAB plus sgRNAs produces no change in interactive strength between Arc promoter and SARE as well as other sites of the gene (n = 5 to 6 rats in each group). (E) Hypothetical model depicting condensed chromatin dynamics produced by dCas9-KRAB targeted at Arc SARE in adult amygdala causing decreased eRNA leading to increased NELF binding at Arc promoter and decreased Arc expression.