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. 2022 May 4;8(18):eabn2911. doi: 10.1126/sciadv.abn2911

Fig. 4. ns- and cryo-EMPEM analysis of polyclonal Fabs from SC donor sera.

Fig. 4.

(A) Representative 2D classes and side and top views of composite figures from ns-EMPEM analysis of polyclonal Fabs from three SC donors complexed with β-CoV spikes. The donor numbers along with the corresponding CoV spikes are indicated above each panel in (A). The Fabs are color-coded on the basis of their epitope specificities as indicated at the bottom left. SARS-CoV-2, OC43, HKU1, and MERS spikes are represented in slate gray, light gray, dark gray, and beige, respectively. Three-dimensional reconstructions displaying potential self-reactive antibodies are shown in gray on the top right corners for both donors 1988 and donor 1999 in complex with SARS-CoV-2 spike. (B) Composite figure showing five unique antibody classes, Fab11 to Fab15 colored in shades of red, to SARS-CoV-2 spike NTD reconstructed using cryo-EMPEM analysis of polyclonal Fabs from donors 1988 and 1989 complexed with SARS-CoV-2–stabilized spikes. (C) Surface representation of SARS-CoV-2 spike showing epitopes of Fabs 11 to 15 from (B) on a single NTD (slate gray) with a zoomed-in view displaying the loop residues comprising each epitope. Loop 144 to 156 with the N149 glycan forms an immunodominant element commonly targeted by Fabs 11 to 14. The sub-epitope colors correspond to each Fab shown in (B).