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. 2022 May 2;221(6):e202111018. doi: 10.1083/jcb.202111018

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© 2022 Hanna et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 8. — The SHIP164 paralog UHRF1BP1 localizes on later endosomal compartments relative to SHIP164. (A) Left: The strucutre of UHRF1BP1 as predicted by the fold prediction algorithm AlphaFold. Middle and Right: Alignment of the core channel structures of SHIP164 (green) and UHRF1BP1 (purple) in two different orientations showing structural similarity. (B) Live fluorescence (inverted grays) image of a COS-7 cell expressing exogenous UHRF1BP1-Halo. Scale bar, 20 µm. (C) Live image of the cytoplasm of a COS-7 cell expressing exogenous UHRF1BP1-Halo (magenta) and the endolysosomal marker LAMP1-GFP (green). Arrowheads indicate SHIP164 accumulation juxtaposed to LAMP1-positive organelles. Scale bar, 5 µm. (D) High-magnification live fluorescence images of COS-7 cell expressing exogenous UHRF1BP1-Halo (magenta) and either LAMP1 or Rab7 (green). The individual channels are shown as inverted grays. Scale bar, 2 µm. (E) Time-series of live fluorescence images of exogenous UHRF1BP1-GFP (magenta) and LAMP1-RFP (green). Arrowheads point to a UHRF1BP1 accumulation undergoing fission. Time, seconds. Scale bar, 1 µm.