Table 2.

Acetylcholine released in response to compounds 3c-j and 3a from A549 cells.

Compound	1/2 IC50 µM	Acetyl choline release (pg/ml)		ACh (pg/ml)
		Choline
		Total	Free
3c	50.8	400.7 ± 4.56	320.6 ± 1.16	80.1 ± 4.7
3d	24.6	377.4 ± 4.84	235.6 ± 1.3	141.8 ± 5.01
3e	26.4	387.4 ± 8.17	307.7 ± 3.44	79.7 ± 8.86
3f	16.1	397.6 ± 8.62	221.5 ± 2	176.1 ± 8.85
3g	19	374.5 ± 6.94	291 ± 2.83	83.5 ± 7.49
3h	21.7	362.9 ± 22	275.1 ± 3.1	87.8 ± 22.21
3i	48.3	351.6 ± 4.24	240.8 ± 1.53	110.8 ± 4.51
3j	28.8	330.7 ± 4.89	240 ± 2.59	90.7 ± 5.53
Sunifiram(3a)	43	402.5 ± 2.58	258.2 ± 1.52	144.3 ± 2.99
Control	–	316.7 ± 1.9	255.7 ± 9.78	61 ± 9.69