Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | trpγ1 | Akitake et al., 2015 | ||
| Genetic reagent (Drosophila melanogaster) | trpγG4 | Akitake et al., 2015 | ||
| Genetic reagent (Drosophila melanogaster) | trpγ1,UAS- trpγ/CyO | Akitake et al., 2015 | ||
| Genetic reagent (Drosophila melanogaster) | trpγ1;g(trpγ) | Akitake et al., 2015 | ||
| Genetic reagent (Drosophila melanogaster) | Dh44Mi | Bloomington Drosophila Stock Center | stock # 1454; RRID:BDSC_1454 | Provided by Y Kim (GIST) and Dr GS Suh (KAIST) |
| Genetic reagent (Drosophila melanogaster) | Dh44-GAL4 | Bloomington Drosophila Stock Center | stock # 1453; RRID:BDSC_1453 | Provided by Y Kim (GIST) and Dr GS Suh (KAIST) |
| Genetic reagent (Drosophila melanogaster) | UAS-hid/CyO | Bloomington Drosophila Stock Center | stock # 65403; RRID:BDSC_65403 | |
| Genetic reagent (Drosophila melanogaster) | UAS-mCD8::GFP | Bloomington Drosophila Stock Center | stock # 5130; RRID:BDSC_5130 | |
| Genetic reagent (Drosophila melanogaster) | UAS-nls::tdtomato | Knapp et al., 2015 | Provided by S Marella | |
| Genetic reagent (Drosophila melanogaster) | UAS-dicer2;UAS-trpγRNAi | Akitake et al., 2015 | Stock # 107656VDRC-107656 | |
| Genetic reagent (Drosophila melanogaster) | iav-GAL4 | Kwon et al., 2010 | ||
| Genetic reagent (Drosophila melanogaster) | dilp2-GAL4 | Rulifson et al., 2002 | Provided by EJ Rulifson | |
| Genetic reagent (Drosophila melanogaster) | Gr64ab | Jiao et al., 2007 | ||
| Genetic reagent (Drosophila melanogaster) | trpA11 | Kwon et al., 2008 | ||
| Genetic reagent (Drosophila melanogaster) | amo1 | Watnick et al., 2003 | ||
| Genetic reagent (Drosophila melanogaster) | trpml2 | Venkatachalam et al., 2008 | ||
| Genetic reagent (Drosophila melanogaster) | trp343 | Wang et al., 2005 | Originally isolated from W Pak lab | |
| Genetic reagent (Drosophila melanogaster) | pyx3 | Lee et al., 2005 | ||
| Genetic reagent (Drosophila melanogaster) | wtrwex | Wang et al., 2005 | ||
| Genetic reagent (Drosophila melanogaster) | trpl29134 | Niemeyer et al., 1996 | ||
| Genetic reagent (Drosophila melanogaster) | pain2 | Tracey et al., 2003 | ||
| Genetic reagent (Drosophila melanogaster) | nan36a | Kim et al., 2003 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-GCaMP6s | Bloomington Drosophila Stock Center | stock # 77131; RRID:BDSC_77131 | |
| Genetic reagent (Drosophila melanogaster) | UAS-dsRed | Bloomington Drosophila Stock Center | stock # 59853; RRID:BDSC_59853 | |
| Genetic reagent (Drosophila melanogaster) | 20xUAS-ASAP2s | Bloomington Drosophila Stock Center | stock# 76247; RRID:BDSC_76247 |
|
| Genetic reagent (Drosophila melanogaster) | UAS-Kir2.1 | Bloomington Drosophila Stock Center | stock # 6596; RRID:BDSC_6596 | |
| Genetic reagent (Drosophila melanogaster) | iav3621 | Bloomington Drosophila Stock Center | stock # 24768; RRID:BDSC_24768 | Generated by Dr P Salvaterra |
| Genetic reagent (Drosophila melanogaster) | tubulin-GAL80ts | Bloomington Drosophila Stock Center | stock # 7017; RRID:BDSC_7017 | |
| Antibody | Mouse anti-GFP (mouse monoclonal) | Molecular Probes | A-11120 | (1:1000, 2 μL) |
| Antibody | Rabbit anti-DsRed (rabbit polyclonal) | Clontech | 632496 | (1:1000, 2 μL) |
| Antibody | Goat anti-mouse IgG (mouse polyclonal) | Thermo Fisher/Invitrogen | A-11031 | Alexa Fluor 488 (1:200, 10 μL) |
| Antibody | Goat anti-rabbit IgG (rabbit polyclonal) | Thermo Fisher/Invitrogen | A-11034 | Alexa Fluor 568 (1:200, 20 μL) |
| Antibody | Rabbit anti-DH44 (rabbit polyclonal) | Cabrero et al., 2002 | (1:500, 15 μL) | |
| Commercial assay or kit | Glucose (HK) Assay Kit | Sigma-Aldrich | Sigma: G3293 | |
| Chemical compound, drug | Brilliant blue FCF | Takeda-Wako Pure Chemical Industry Ltd | Cat. 3844-45-9 | |
| Chemical compound, drug | Paraformaldehyde | Electron Microscopy Sciences | Cat. 15710 | |
| Chemical compound, drug | Sulforhodamine B | Sigma-Aldrich | Cat. 3520-42-1 | |
| Chemical compound, drug | Sucrose | Sigma-Aldrich | Cat. 57-50-1 | |
| Chemical compound, drug | D-(+)-Glucose | Sigma-Aldrich | Cat. 50-99-7 | |
| Chemical compound, drug | D-(-)-Fructose | Sigma-Aldrich | Cat. 57-48-7 | |
| Chemical compound, drug | D-(+)-Maltose | Sigma-Aldrich | Cat. 6363-53-7 | |
| Chemical compound, drug | D-(+)-Trehalose | Sigma-Aldrich | Cat. 6138-23-4 | |
| Chemical compound, drug | KCl | Sigma-Aldrich | Cat. 7447-40-7 | |
| Chemical compound, drug | Hydrochloric acid | Samchun | Cat. 7647-01-0 | |
| Chemical compound, drug | Ethanol | Merck | Cat. 64-17-5 | |
| Chemical compound, drug | Trehalase from porcine kidney | Sigma-Aldrich | Cat. 9025-52-9 | |
| Chemical compound, drug | Amyloglucosidase from Aspergillus | Sigma-Aldrich | Cat. 9032-08-0 |