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. 2022 Mar 28;11:e71227. doi: 10.7554/eLife.71227

Figure 2. Swimming trajectories of P. parasitica zoospores.

Figure 2.

(A) Trajectories of zoospores swimming in water captured from the microscopic assay for 60 s. Sample size N=58. Note: not all trajectories are shown. Each position of the zoospores is captured every Δt=0.0167s. The trajectories are smoothed with moving average (step length n=12). (B) The progression of speed U and (C) moving directions θ over time of a single zoospore extracted from the population in the assay. (D) Distribution of zoospore speed p(U). (E) Polarity distribution of moving direction p(θ). (F) Survival curves p(τt) of the running time τr and stopping time τs. (G) Distribution of turning angle p(Δθ), with positive angle values indicating counter-clockwise, and negative as clockwise direction. (H) Schematics showing the strategy of the simulation model of zoospores swimming in water. (I) The estimated mean squared displacement (MSD) over time intervals t, constructed from the simulation data. The inset compares the experimental data and simulation of MSD at the experimental time-scale of 60 s. By simulation, at long time scale of 1 h, MSD of zoospores shows a diffusion of Brownian particles with the diffusion coefficient D=3.5×10-4cm2s-1.