| Strain, strain background (Escherichia coli) |
E.cloni 10G |
Lucigen |
60107 |
High-transformation efficiency electrocompetent cells |
| Cell line(Mus musculus) |
BW5147 |
Kind gift from McClusky lab |
CVCL_3896 |
|
| Cell line(Homo sapiens) |
SKBR3 |
Kind gift from Jenkins lab |
CVCL_0033 |
|
| Cell line(M. musculus) |
MC57 |
Kind gift from Jenkins lab |
CVCL_4985 |
|
| Cell line(M. musculus) |
MC57-HER2 |
Kind gift from Jenkins lab |
CVCL_4985 |
|
| Cell line(M. musculus) |
MC38-HER2 |
Kind gift from Jenkins lab |
CVCL_B288 |
|
| Cell line(H. sapiens) |
HEK293T |
Cellbank Australia |
Cat# 12022001 |
|
| Antibody |
Pacific Blue anti-mouse CD69 Armenian hamster IgG monoclonal antibody(clone: H1.2F3) |
BioLegend |
Cat# 104524 |
Flow cytometry (1:200) |
| Antibody |
PE anti-mouse CD3ε Armenian hamster IgG monoclonal antibody(clone: 145-2C11) |
BioLegend |
Cat# 100308 |
Flow cytometry (1:800) |
| Antibody |
APC-Cyanine7 anti-mouse CD8α rat IgG2a, κ monoclonal antibody(clone: 53-6.7) |
BioLegend |
Cat# 100714 |
Flow cytometry (1:800) |
| Antibody |
Alexa Fluor 647 anti-human c-Myc-tag mouse IgG2a monoclonal antibody(clone: 9B11) |
Cell Signaling |
Cat# 2233S |
Flow cytometry (neat) |
| Antibody |
APC anti-mouse CD28 Syrian hamster IgG monoclonal antibody(clone: 37.51) |
BioLegend |
Cat# 102110 |
Fluorescence microscopy (1:400) |
| Antibody |
Anti-human c-Myc-tag mouse IgG2a monoclonal antibody(clone: 9B11) |
Cell Signaling |
Cat# 2276 |
IP (1:2000)Fluorescence microscopy (1:100) |
| Antibody |
HRP anti-mouse IgG polyclonal antibody |
Sigma-Aldrich |
Cat# A0168 |
IP (1:20,000) |
| Antibody |
Alexa Fluor 488 goat anti-mouse IgG polyclonal antibody |
Abcam |
Cat# ab150113 |
Fluorescence microscopy (1:200) |
| Antibody |
LEGENDplex mouse cytokine panel 2 detection antibodies |
BioLegend |
Cat# 740149 |
(1:4)Reagent used for cytokine detection in Figures 3, 4 and 6
|
| Antibody |
LEGENDplex MU Th panel detection antibodies V02 |
BioLegend |
Cat# 741045 |
(1:4)Reagent used for cytokine detection in Figures 3, 4 and 6
|
| Antibody |
Alexa Fluor647 anti-human CD340 (erbB2/HER2) mouse IgG1, κ antibody(clone: 24D2) |
BioLegend |
Cat# 324412 |
Flow cytometry (1:3200) |
| Recombinant DNA reagent |
pMAL-dsTbL(plasmid) |
Elazar et al., 2016a
|
Addgene:73805 |
TOXCAT β-lactamase assays |
| Recombinant DNA reagent |
Designed proCARs |
This paper |
|
See Experimental Methods - DNA sequences of designs |
| Sequence-based reagent |
Deep sequencing primers |
Elazar et al., 2016a
|
|
Deep sequencing library preparation and a protocol and analysis as described |
| Peptide, recombinant protein |
Retronectin |
Takara Bio |
Cat# T100B |
Final concentration (32 μg/ml) |
| Peptide, recombinant protein |
Recombinant mouse IL-2 (ELISA Std.) |
BioLegend |
Cat# 575409 |
|
| Peptide, recombinant protein |
Recombinant mouse TNFα (ELISA Std.) |
BioLegend |
Cat# 575209 |
|
| Peptide, recombinant protein |
Recombinant mouse IFNγ (ELISA Std.) |
BioLegend |
Cat# 575309 |
|
| Peptide, recombinant protein |
Recombinant mouse GM-CSF (ELISA Std.) |
BioLegend |
Cat# 576309 |
|
| Peptide, recombinant protein |
Recombinant human IL-2 |
PeproTech |
Cat# 200-02-1 |
Dose(5 × 104 IU/injection)Media concentration(100 IU/ml) |
| Commercial assay or kit |
Mouse T-activator CD3/CD28 Dynabeads |
Gibco |
Cat# 11456D |
|
| Commercial assay or kit |
EasySep mouse CD8a positive kit II |
Stem Cell Technologies |
Cat# 18953 |
|
| Chemical compound, drug |
Roswell Park Memorial Institute (RPMI) 1640 Medium +Pen/Strep |
Gibco |
In-house |
|
| Chemical compound, drug |
Dulbecco′s Modified Eagle Medium (DMEM) |
Lonza |
Cat# BE12-707F |
|
| Chemical compound, drug |
Dulbecco′s phosphate buffered saline |
Gibco |
In-house |
|
| Chemical compound, drug |
Fetal bovine serum |
Bovogen Biologicals |
Cat# 423101 |
Final concentration (10% v/v) |
| Chemical compound, drug |
Polybrene |
Sigma-Aldrich |
TR-1003-G |
Final concentration (8 µMg/ml) |
| Chemical compound, drug |
Zombie Aqua |
BioLegend |
Cat# 423101 |
Flow cytometry (1:500) |
| Chemical compound, drug |
Propidium iodide |
Sigma-Aldrich |
Cat# P4170 |
Final concentration (50 µM) |
| Chemical compound, drug |
l-Glutamine |
Gibco |
Cat# 25030081 |
Final concentration (2 mM) |
| Chemical compound, drug |
Sodium pyruvate |
Gibco |
Cat# 11360070 |
Final concentration (1 mM) |
| Chemical compound, drug |
Non-essential amino acids |
Gibco |
Cat# 11140050 |
Final concentration (1×) |
| Chemical compound, drug |
β-Mercaptoethanol |
Sigma-Aldrich |
Cat# M3148 |
Final concentration (50 µM) |
| Chemical compound, drug |
Saponin |
Sigma-Aldrich |
Cat# 47036 |
Final concentration (1.2% w/v) |
| Chemical compound, drug |
Sodium azide |
Sigma-Aldrich |
Cat# 71289 |
Final concentration (0.1% w/v) |
| Software, algorithm |
GraphPad Prism v9.0 |
GraphPad Software |
|
|
| Software, algorithm |
FlowJo v10 |
FlowJo Software |
|
|
| Software, algorithm |
IncuCyte Analysis Software |
IncuCyte Analysis Software |
|
|
| Other |
Rosetta macromolecular modeling suite |
Rosetta |
Git version: b210d6d5a0c21208f4f874f62b2909f926379c0f |
For documentation, see https://www.rosett acommons.org/
|
| Other |
Cr51
|
PerkinElmer |
|
100 µCi |
| Other |
LEGENDplex mouse IL-2 capture bead A7 |
BioLegend |
Cat# 740054 |
Reagent used for cytokine detection in Figures 3, 4 and 6
|
| Other |
LEGENDplex mouse TNFα capture bead A6 |
BioLegend |
Cat# 740066 |
Reagent used for cytokine detection in Figures 3, 4 and 6
|
| Other |
LEGENDplex IFNγ capture bead A4 |
BioLegend |
Cat# 740065 |
Reagent used for cytokine detection in Figures 3, 4 and 6
|
| Other |
LEGENDplex GM-CSF capture bead B7 |
BioLegend |
Cat# 740146 |
Reagent used for cytokine detection in Figures 3, 4 and 6
|
