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ZQL-4c induces reactive oxygen species production in breast cancer cells. MCF-7, MDA-MB-231, and SK-BR-3 cells were treated with 0, 0.4, 0.8, and 1.6 μmol/L ZQL-4c for 24 h. Cells were loaded with 10 μmol/L DCFH-DA for 20 min. The nuclei were stained with Hoechst 33342. The level of ROS production was detected by fluorescence microscopy (a) and flow cytometry (b).
