TABLE 1.

Summary of RNA-seq studies in Ulcerative Colitis.

	Broad study	Oxford study
Study subjects	18 patients with UC and 12 healthy controls	3 patients with UC and 3 healthy individuals
Biopsy samples	One non-inflamed and one inflamed region or Two adjacent non-inflamed and two adjacent inflamed biopsies	Endoscopically inflamed distal areas of the colon and proximal clinically non-involved regions
Number of cells yielded	366,650 cells	11,175 cells
Single cell suspension	Dissociation with TrypLE Express Enzyme	Dissociation with TrypLE Express Enzyme
RNA-seq methodology	Droplet-based scRNA-seq technique	Droplet-based scRNA-seq technique
Library preparation	SMART-Seq2 protocol	SMART-Seq2 protocol
Differential gene expression	MAST; AUC ≥ 0.75	Seurat; AUC ≥ 0.7
Cells identified	Identified fifteen distinct groups of epithelial cells: - Stem cells - TA 1 - TA 2 - Cycling TA - Immature Enterocytes 1 - Immature Enterocytes 2 - Enterocyte Progenitors - Enterocytes - Microfold cells - BEST4 + Enterocytes - Secretory TA - Immature Goblet cells - Goblet cells - Tuft cells - Enteroendocrine cells	Identified seven distinct groups of epithelial cells*: - Undifferentiated #1 - Undifferentiated #2 - Enteroendocrine cells – Colonocytes - Crypt Top Colonocytes - BEST4 + /OTOP2 cells - Goblet cells

TA: transit amplifying.

*Which were further subdivided into 5 clusters based on their location along crypt-surface axes.