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. 2022 Apr;11(4):687–701. doi: 10.21037/gs-22-29

Figure 4.

Figure 4

ASPM promoted cell proliferation in OVCAR3 and A2780 cells. (A,B) The proliferation capacity of OVCAR3 and A2780 cells was estimated by the CCK-8 assay. (C,D) The positive rate of cell proliferation of OVCAR3 and A2780 cells by EdU assay. The magnification is ×200. (E,F) Cell proliferation rate in OVCAR3 and A2780 cells was calculated by ImageJ. (G) Effect of ASPM on colony formation in OVCAR3 and A2780 cells stained by 2% crystal violet. The magnification is 0. (H-I) Quantitative changes in OVCAR3 and A2780 colony formation were evaluated by counting using ImageJ. Data represent the mean ± SD of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. OVCAR3, human ovarian adenocarcinoma cell 3; SD, standard deviation; OD, optical density; CCK-8, cell counting kit-8.