Fig. 4.

MiR-100 interacted with HAGLROS in NSCLC. (A and B) The changes of miR-100 expression were examined by RT-PCR and Northern blot when HAGLROS was overexpressed or knocked down in NSCLC cells. (C) Dual-luciferase reporter assay was done to check the luciferase activity of cells to verify the binding relationship between HAGLROS and miR-100. (D) RT-PCR was carried out to explore the correlation between HAGLROS expression and miR-100 expression in 54 cases of NSCLC samples. (E) MTT assay was used to monitor the proliferation of A549 cells co-transfected with HAGLROS overexpression plasmids and miR-100 mimics. (F and G) Transwell assay was performed to monitor the migration and invasion of A549 cells co-transfected with HAGLROS overexpression plasmids and miR-100 mimics. ∗∗p < 0.01, NS: p > 0.05.