Fig. 3.

Residue-specific amide chemical shift perturbation (CSP) for Ca²⁺-bound CaM in the presence and absence of CNGB1 peptide. CSP was calculated as: $CSP=\sqrt{{\left(\mathrm{\Delta },H^N\right)}^2+{\left(\mathrm{\Delta },N\right)}^2}$. ΔH^N and ΔN are the observed difference in the ¹H^N and ¹⁵N chemical shifts, respectively for CaM/CNGB1 compared to CaM alone. CSP values are mapped on to the CaM structure (PDB ID: 2VAY (Halling et al. 2009))