Table 2.
Echocardiographic measurements in anesthetized mice
| Echocardiographic Parameters | Age | Stimulation | Genotype | Numbers (n) | |||
|---|---|---|---|---|---|---|---|
| CSQ2(+/+) | CSQ2(−/−) | ||||||
| Mean | SEM | Mean | SEM | ||||
| Aorta descendens peak velocity (mm/s) | Adult | Ctr | 950.61 | 28.31 | 906.12 | 60.21 | 8/7 |
| Iso | 1,017.67 | 58.26 | 1,144.29+# | 49.97 | 3/4 | ||
| Young | Ctr | 920.36 | 34.46 | 960.36 | 57.97 | 6/7 | |
| Iso | x | x | 880.15 | 23.90 | 0/2 | ||
| Aorta descendens VTI (mm) | Adult | Ctr | 29.27 | 1.35 | 27.18# | 1.30 | 8/7 |
| Iso | 25.41 | 2.03 | 25.91 | 1.65 | 3/4 | ||
| Young | Ctr | 29.77 | 1.71 | 32.23 | 1.68 | 6/7 | |
| Iso | x | x | 28.09 | 0.98 | 0/2 | ||
| Pulmonary artery peak velocity (mm/s) | Adult | Ctr | 711.40 | 31.94 | 591.18 | 56.14 | 8/7 |
| Iso | 845.89 | 57.70 | 879.39+ | 29.06 | 3/4 | ||
| Young | Ctr | 794.85 | 28.79 | 688.91* | 32.29 | 6/7 | |
| Iso | 794.98 | 49.45 | 838.00+ | 34.40 | 6/7 | ||
| Pulmonary artery VTI (mm) | Adult | Ctr | 25.79 | 0.67 | 20.68*# | 1.08 | 8/7 |
| Iso | 22.20 | 2.54 | 23.89 | 0.85 | 3/4 | ||
| Young | Ctr | 29.92 | 1.08 | 26.30 | 1.10 | 6/7 | |
| Iso | 25.17 | 1.69 | 25.40 | 1.15 | 6/7 | ||
| Ejection fraction | Adult | Ctr | 76.46 | 1.99 | 62.51* | 4.26 | 8/7 |
| Iso | 90.82+ | 1.61 | 71.75*# | 2.89 | 8/7 | ||
| Young | Ctr | 78.77 | 1.85 | 74.50 | 4.15 | 6/7 | |
| Iso | 90.75+ | 2.62 | 89.51+ | 2.60 | 6/7 | ||
| Heart rate (BPM) | Adult | Ctr | 496.72 | 9.79 | 432.90* | 32.07 | 8/7 |
| Iso | 550.56+ | 4.41 | 532.40+ | 14.17 | 8/7 | ||
| Young | Ctr | 502.26 | 26.66 | 421.65 | 30.68 | 6/7 | |
| Iso | 557.75 | 34.41 | 554.81+ | 16.86 | 6/7 | ||
| Left ventricular end-diastolic diameter (mm) | Adult | Ctr | 3.22 | 0.16 | 3.31 | 0.13 | 8/6 |
| Iso | 2.78 | 0.12 | 2.90+ | 0.10 | 6/6 | ||
| Young | Ctr | 2.99 | 0.11 | 3.15 | 0.19 | 6/7 | |
| Iso | 2.54 | 0.21 | 2.54 | 0.21 | 6/7 | ||
| Left ventricular end-systolic diameter (mm) | Adult | Ctr | 1.62 | 0.05 | 2.18* | 0.13 | 6/7 |
| Iso | 0.96+ | 0.09 | 1.59*+# | 0.11 | 6/7 | ||
| Young | Ctr | 1.63 | 0.07 | 1.82 | 0.20 | 6/7 | |
| Iso | 0.95+ | 0.16 | 1.05+ | 0.17 | 6/7 | ||
Genotype indicates CSQ2(+/+) and CSQ2(−/−) mice. Tissue Eʹ/Aʹ indicates tissue Doppler data where the Eʹ-value is divided by the Aʹ- value. Adult means mice about 18 months old and young means about six months old. See Table 1 for exact ages. Numbers of animals are indicated in the last column. X = no stable echocardiographic recordings could be obtained. Here, only parameters with differences between genotypes are shown. For the complete data table see supplementary table 1
*p < 0.05 vs. CSQ2(+/+)
#p < 0.05 vs. young age group of each genotype
+p < 0.05 vs. control (Ctr). Ctr means control condition that is before β-adrenergic stimulation by injection of isoproterenol. Iso means values after injection of isoproterenol