Table 4.
Immunological parameters
| Immune parameters | |||||
|---|---|---|---|---|---|
| Age | Young | Adult | Numbers of mice | ||
| Genotype | CSQ2(+/+) | CSQ2(−/−) | CSQ2(+/+) | CSQ2(−/−) | Young; Adult |
| FACS | |||||
| Mediastinal Lymph Nodes | |||||
| cells/LN | 114,756 ± 26,300 | 256,597 ± 118,998 | 58,846 ± 20,507 | 257,067 ± 94,118 | 3/3; 6/5 |
| Frequency of B cells | 24 ± 4.73 | 30 ± 3.82 | 26 ± 4.32 | 38 ± 5.08 | 3/3; 6/5 |
| Frequency of CD 4 + CD44 + cells | 10 ± 4.14 | 12 ± 1.37 | 43 ± 3.56# | 31 ± 5.36 | 3/3; 6/5 |
| Frequency of CD 8 + CD44 + cells | 11 ± 3.93 | 11 ± 1.41 | 55 ± 11.71# | 41 ± 13.24 | 3/3; 6/5 |
| Heart | |||||
| Frequency of T cells | 27.4 ± 1.11 | 21.1 ± 1.56 | 7.91 ± 1.94# | 10.48 ± 3.36 | 3/3; 6/5 |
| Frequency of B cells | 32 ± 2.04 | 27.67 ± 2.46 | 11.96 ± 4.73# | 12.39 ± 3.01# | 3/3; 5/5 |
| Immunofluorescence monoclonal antibodies | |||||
| Anti-IgM | 22,455 ± 4,457 | 18,800 ± 2,878 | 34,057 ± 5,158 | 29,300 ± 4,565 | 6/5; 3/5 |
| Anti-IgG | 19,808 ± 3,066 | 25,407 ± 1,339 | 26,923 ± 2,559 | 25,192 ± 2,409 | 6/5; 3/5 |
| DAPI | 91,735 ± 6,582 | 76,384 ± 12,454 | 67,490 ± 9,090 | 63,082 ± 10,702 | 6/5; 3/5 |
Using fluorescence-activated cell sorting (FACS) with FlowJo Single-Cell Analysis Software leucocytes in the samples were analyzed. Mean intensity of immunofluorescence signals using an anti-mouse IgG, IgM, and DAPI conjugated with fluorescent dyes was measured using a Zeiss Axioskop 2 Plus and ImageJ software. Genotype indicates CSQ2(+/+) and CSQ2(−/−) mice. Adult means mice about 18 months old and young means about six months old. See Table 1 for exact ages. Numbers of animals are indicated in the last column
#p < 0.05 vs. young age group of each genotype. FACS fluorescence-activated cell sorting, LN lymph nodes, Anti-IgM anti-immunoglobulin M antibody, Anti-IgG anti-immunoglobulin G antibody, DAPI 2-[4-(aminoiminomethyl)phenyl]-1HiIndole-6-carboximidamide hydrochloride (for labeling of nuclei)