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. 2022 May 4;13(5):435. doi: 10.1038/s41419-022-04886-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Western blotting was performed to evaluate the expression of P16, P21, TERT, αSMA and Collagen-I in IPF lung tissue and control lung tissues. Four randomly selected sample from each group were shown. B, D qPCR were performed to test P16, P21 and TERT in human IPF lung tissues and control group. C Double-labeled immunofluorescent staining were performed to examine the expression of SP-C (green) and P21 (red) in AECs. E Quantification of the protein level of TERT. F Double-labeled immunofluorescent staining were performed to examine the expression of SP-C (green) and TERT (red) in AECs. G, H qPCR and western blotting were performed to test KLF4 expression level in human IPF lung tissues and control group. I Immunofluorescent staining were performed to examine the expression of SP-C (green) and KLF4 (red) in AECs. *P < 0.05, **P < 0.01, ***P < 0.001 by t-test.