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. 2022 May 4;8:244. doi: 10.1038/s41420-022-00872-2

Fig. 2. Overexpression of WWP1 promotes NLRP3 ubiquitination to inactivate NLRP3 inflammasomes and caspase-1-mediated cleavage of GSDMD.

Fig. 2

A The expression of WWP1 detected by RT-qPCR. B The expression of TNF-α and IL-1βdetected by ELISA. C LDH level in cells with different treatment. D The activation of NLRP3 inflammasomes detected by Western blot assay. E The co-localization of ASC with pro-caspase-1 and caspase-1, respectively in macrophages detected by immunofluorescence. F Protein levels of GasD-N and GasD-FL detected by Western blot assay. G The protein expression of NLRP3 in oe-WWP1-treated cells in response to MG132 treatment. H The ubiquitination of NLRP3 detected by Co-IP (WWP1: 105 kDa; NLRP3: 118 kDa). I K63 ubiquitination and K48 ubiquitination of NLRP3 detected by Co-IP (WWP1: 105 kDa; NLRP3: 118 kDa). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ‘ns’ indicates no significant difference. Cell experiments were repeated three times.