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. 2022 May 4;8:244. doi: 10.1038/s41420-022-00872-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A The expression of WWP1 and four m6A readers in sepsis-related microarray GSE00159 (measurement data were expressed as mean ± standard deviation, and paired samples t test was applied for comparison between the control group and the sepsis group). B RT-qPCR to detect the expression of YTHDF1 in PBMCs of sepsis samples (n = 40) and healthy controls (n = 40). C The expression of YTHDF1 in PBMCs of mice with different treatment detected by RT-qPCR. D The expression of YTHDF1 determined by RT-qPCR. E The expression of YTHDF1 and WWP1 in cells with different treatment assessed by RT-qPCR. F Western blot assay to measure the protein expression of YTHDF1 and WWP1 in cells with different treatment. G The m6A modification status of WWP1 mRNA evaluated by meRIP. H, RIP-RT-qPCR to assess the binding of WWP1 mRNA to YTHDF1 protein. I Dual luciferase gene reporter assay to evaluate the binding of WWP1 and YTHDF1. There were 10 mice in each group. *p < 0.05; **p < 0.01; ***p < 0.001. ****p < 0.0001. ‘ns’ indicates no significant difference. Cell experiments were repeated three times.