Fig. 5. Difluorosamine reduces Th17 population in the CNS during EAE.

a A CSPG-lowering drug, difluorosamine (DIF), was administered at 25 mg/kg to mice daily starting three days post onset of EAE for 5 days. Data are presented as mean ± SEM of n = 10 mice per group pooled from 2 independent experiments. Results were analyzed using two-way repeated-measures ANOVA with Sidak’s post-hoc test; *p = 0.02. Average EAE clinical score of mice is shown with treatment period indicated by green box. b The frequency of different T cell subsets was determined using flow cytometry. c Bar graphs showing the frequency of CD3⁺, CD4⁺ and CD8⁺ cells; and d IFN-γ⁺, IL-17⁺ or FOXP3⁺ T cells within the CD4⁺ or CD8⁺ populations in the spinal cord and lymph nodes of DIF or vehicle treated mice. Data are presented as mean ± SEM. n = 4 mice per group for c, d, and results were reproduced in a second experiment (source data file). Two-tailed unpaired Student’s t test; **p = 0.003 for c, and *p = 0.025 or **0.0017 for d. Source data are provided in the source data file.