Fig. 8. Difluorosamine reduces versican-V1 expression and promotes presumed remyelination in EAE.

Longitudinal sections of the thoracic spinal cord from EAE NG2^CreER:MAPT^mGFP mice following DIF or vehicle treatment were analyzed. a Examples of slide scanner images showing lesions defined by CD45⁺ accumulation; the dotted rectangles are correspondent with high magnification images in b acquired by confocal microscopy (Z-stack). c Bar graph (n = 4 mice per group) comparing the extent of GFP⁺ in the white matter (WM), expressed as % of GFP immunoreactivity of the whole thoracic section. d Bar graph of GFP⁺ in region of interest (ROI), where each ROI is a lesion defined by area occupied by CD45⁺ cell (n = 30 ROIs from 4 mice per group). e GFP⁺ cells. f Bar graph comparing average particle size of GFP in each CD45⁺ lesional ROI (n = 41 ROIs from 4 mice per group. h Representative immunofluorescent images (z-stack) labeled for GFP (green), olig2 (white) and CC1 (red). g, i, j Bar graphs comparing the number of olig2⁺, mature oligodendrocytes (olig2⁺CC1⁺) and GFP⁺ oligodendrocytes in the white matter as defined in c (n = 4 mice). k Representative images (z-stack) labeled with GFP (green), versican-V1 (red) and stub chondroitin-4-sulfate (gray). l, m Quantification of the percent area occupied by immunoreactive CSPGs or versican-V1 in lesional CD45⁺ ROI (n = 4 mice). Data presented as mean ± SEM from one experiment, n = 4 mice in each group, each dot represents one mouse where a mean of 8 lesions were analyzed per mouse, two-tailed unpaired Student’s t test. This data was reproduced in a second experiment. n Correlation analysis was performed between percent of GFP⁺ and CSPGs⁺ in the lesion ROI. Each dot represents one ROI from 4 mice per group. Pearson r –0.39; *p = 0.029. Source data are provided in the source data file.