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. 2022 May 4;12:7302. doi: 10.1038/s41598-022-10776-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) and (B) Calculated growth rate in absorbance increase per hour as determined by the first derivative of a growth curve for streptomycin and tobramycin respectively. Samples from the growth assay are processed and ran on a polyacrylamide gel where the pixel density of LPS bands were determined over the various concentrations and plotted as sigmoid curves for streptomycin (C) and tobramycin (D). IC₅₀ values were determined from the inflection point of these fittings at 6.4 ± 0.1 and 26.2 ± 1.0 ng/mL for tobramycin and streptomycin respectively.