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Experimental design. A Animal preparation for the SNI model and implantation of the SCS electrode and intrathecal catheter. B Procedure for 30-min SCS. C SCS at different intensities. D Short-term SCS at different frequencies and collection of CSF. E 24-h SCS at different frequencies and collection of CSF. SCS, spinal cord stimulation; PWT, paw withdrawal threshold; SNI, spared nerve injury; MT, motor threshold; CSF, cerebrospinal fluid.
