FIGURE 1.

The expression levels of the P2Y₁₄ receptor and GFAP in SCG. The protein expression levels of P2Y₁₄ and GFAP were assessed by Western blotting (A). The bar histogram shows the integrated optical density (IOD) ratio of P2Y₁₄ and GFAP protein mass to β-actin protein mass (actin as an internal control for normalization) in each group (B), and the values are the mean ± SEM from three independent experiments. ***p < 0.001 vs. Ctrl; ###p < 0.001 vs. DM. The expression of P2Y₁₄, GFAP, and DAPI in the SCG was detected by immunofluorescence staining (C). The red signal indicates P2Y₁₄ staining with TRITC, the green signal represents GFAP staining with FITC, and the blue signal shows DAPI stain for labeling DNA. The merged image represents the three staining of P2Y₁₄, GFAP, and DAPI. Scale bar, 20 μm.