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. 2022 May 4;13(5):433. doi: 10.1038/s41419-022-04805-w

Fig. 2. TUG1 loss-of-function leads to suppression of CRC cell viability and invasion and promotion of cell apoptosis.

Fig. 2

A Determination of TUG1 expression in HCT116 and SW480 cells transfected with si-TUG1-1 and si-TUG1-2 by RT-qPCR. B–D Assessment of HCT116 and SW480 cell viability (B), invasion (C), and apoptosis (D) by CCK-8 assay, Transwell assay (200×) and flow cytometry, respectively following transfection with si-TUG1-1. E Western blot assay of PARP, cleaved PARP, Caspase-3, and cleaved Caspase-3 proteins in HCT116 and SW480 cells following transfection with si-TUG1-1. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, compared with HCT116 and SW480 cells transfected with si-NC. The measurement data are expressed as mean ± standard deviation. The experiments were repeated three times independently. Comparison between two groups was conducted by unpaired t-test. Multi-group comparison was conducted by one-way ANOVA with Tukey’s post hoc test.