Table 3.
Using ImageJ to evaluate and calculate the average pixel intensity and the percentage of the positive staining pattern of the IHC images.
| Antibody | Gleason score | Area | Staining area % | The mean of staining area | Location of staining (nuclei or fibromuscular stroma) |
|---|---|---|---|---|---|
| FAP | 3+3 | 4194304 | 9.8 | 7.9 | Nuclei |
| 3+4 | 4194304 | 2.1 | 17.5 | Nuclei | |
| 4+3 | 4194304 | 4.5 | 18.3 | Nuclei | |
| 3+5 | 4194304 | 15.7 | 44.5 | Nuclei | |
| 4+5 | 4194304 | 15.4 | 56.0 | Nuclei | |
| SMAα | 3+3 | 4194304 | 5.1 | 9.2 | Fibromuscular stroma |
| 3+4 | 4194304 | 7.7 | 19.4 | Fibromuscular stroma | |
| 4+3 | 4194304 | 16.7 | 35.3 | Fibromuscular stroma | |
| 3+5 | 4194304 | 21.7 | 40.1 | Fibromuscular stroma | |
| 4+5 | 4194304 | 25.7 | 45.1 | Fibromuscular stroma | |
| FSP1 | 3+3 | 4194304 | 6.9 | 6.6 | Fibromuscular stroma |
| 3+4 | 4194304 | 8.8 | 8.4 | Fibromuscular stroma | |
| 4+3 | 4194304 | 8.7 | 16.9 | Fibromuscular stroma | |
| 3+5 | 4194304 | 9.6 | 25.4 | Fibromuscular stroma | |
| 4+5 | 4194304 | 9.7 | 21.9 | Fibromuscular stroma | |
| CD36 | 3+3 | 4194304 | 0.7 | 2.4 | Nuclei |
| 3+4 | 4194304 | 2.8 | 18.4 | Nuclei | |
| 4+3 | 4194304 | 2.2 | 7.5 | Nuclei | |
| 3+5 | 4194304 | 14.6 | 35.2 | Nuclei | |
| 4+5 | 4194304 | 0.7 | 0.8 | Nuclei | |
| PDGFRα | 3+3 | 4194304 | 0.5 | 2.6 | Nuclei |
| 3+4 | 4194304 | 7.9 | 26.4 | Both | |
| 4+3 | 4194304 | 14.3 | 26.6 | Both | |
| 3+5 | 4194304 | 11.5 | 44.1 | Both | |
| 4+5 | 4194304 | 12.5 | 44.7 | Both | |
| PDGFRβ | 3+3 | 4194304 | 1.4 | 3.9 | Fibromuscular stroma |
| 3+4 | 4194304 | 8.3 | 30.3 | Fibromuscular stroma | |
| 4+3 | 4194304 | 11.3 | 39.3 | Fibromuscular stroma | |
| 3+5 | 4194304 | 15.8 | 59.0 | Fibromuscular stroma | |
| 4+5 | 4194304 | 25.4 | 96.5 | Fibromuscular stroma |