Table 2.
Droplet digital PCR results on singleplex and tetraplex assay in copies/μl on a 10-fold dilution series of NZ-RLO1, NZ-RLO2, Tenacibaculum maritimum, and Yersinia ruckeri gBlocks™ starting from 10 ng/μl.
| gBlock™ concentration (ng/μl) | Singleplex (copies/μl) | Tetraplex (copies/μl) | ||||||
|---|---|---|---|---|---|---|---|---|
| NZ-RLO1 | NZ-RLO2 | Tenacibaculum maritimum | Yersinia ruckeri | NZ-RLO1 | NZ-RLO2 | Tenacibaculum maritimum | Yersinia ruckeri | |
| 10 | >106 | >106 | >106 | >106 | >106 | >106 | >106 | >106 |
| 1 | >106 | >106 | >106 | >106 | >106 | >106 | >106 | >106 |
| 0.1 | >106 | >106 | >106 | >106 | >106 | >106 | >106 | >106 |
| 0.01 | >106 | >106 | >106 | >106 | >106 | >106 | >106 | >106 |
| 10−3 | >106 | >106 | >106 | >106 | >106 | >106 | >106 | >106 |
| 10−4 | >106 | >106 | >106 | >106 | >106 | >106 | >106 | >106 |
| 10−5 | 6,285 | 6,113 | 8,115 | 1,088 | 695 | 70.6 | 5,289 | 1,118 |
| 10−6 | 337 | 386 | 1,062 | 162 | 163 | 0.8 | 582 | 5.49 |
| 10−7 | 37.2 | 24.7 | 59.9 | 5.38 | 10.3 | 1.06 | 25.1 | 1.43 |
| 10−8 | 3.14 | 3.2 | 5.14 | 1.00 | 0.84 | 0 | 25.9 | 0.07 |
| 10−9 | 0.931 | 0.162 | 0.349 | 0 | 0.14 | 0.21 | 0.93 | 0 |
| 10−10 | 0 | 0 | 0 | 0.08 | 0 | 0 | 0 | 0 |
>106 = too high template concentration to quantify copy numbers.