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Protein production from synthesized reference and virome sequences. SDS-PAGE gel of 6xHis-tagged proteins from reference and virome clones. Red boxes indicate protein bands of interest (expected PolA size ∼64–75 kDa). Prefix of clone names indicate the PolA 762 residue and clade (Figure 1): phenylalanine group (F), leucine clades (L1 or L2), or tyrosine clade (Y); and the character following the underscore indicates the source: reference sequence (R) or virome sequence (S).
