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In vitro primer extension and strand displacement in two synthesized virome polymerases. (A) F_S24 and (B) F_S21A at decreasing (left to right) concentrations (μg/reaction) in 1X phi29 buffer after 18.5 h incubation at 30°C. Both polymerases demonstrated primer extension and strand displacement from both single-stranded M13 (left) and double-stranded M13 (right) DNA templates. Strand displacement is evident in the phi29 DNA polymerase positive control after 10 min incubation at 37°C.
