Figure 8.

Targeting of CD146 with functional antibody AA98 inhibits VAT inflammation in diet‐induced obesity. A) Body weights of C57 mice fed with ND (n = 6) or HFD (n = 25). HFD mice were treated with AA98 antibody or control mIgG (n = 8 per group). Concentrations of B) sera TG and FAA and C) glucose in ND‐fed (n = 5) and HFD‐fed mice treated with AA98 (10 mg kg⁻¹, n = 6) or mIgG (10 mg kg⁻¹, n = 7) for 12 weeks. D) GTT and E) ITT of ND‐ and HFD‐fed mice treated with AA98 or mIgG (n = 6 each). The histograms on the right show the corresponding AUCs. F) Immunoblotting analysis of AKT, p‐AKT, ERK, p‐ERK, and β‐actin of VAT from antibody‐treated mice intraperitoneally injected with 1 U kg⁻¹ insulin for 10 min (representative of n = 3). G,H) Cell numbers of infiltrated leukocytes in VAT from ND‐fed (n = 5) and antibody‐treated mice (n = 7 for mIgG or 5 for AA98 group). Each symbol represents an individual mouse (B, C, G, and H) or an experiment (D and E); the short horizontal lines indicate the mean ± SEM (B, C, G, and H) or SD (D and E). Two‐way ANOVA with multiple‐comparison test (A, D and E) or one‐way ANOVA (B, C, D, E, G, and H) was performed to test statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001.