Figure 2.
Plot of immediate and serial chase over 3 weeks in vitro. BrdU-positive keratinocytes per field in vitro. Counts of BrdU-positive keratinocytes showed progressive loss of label, whether pulsed at incubation days 5–7, and chased serially at days 8, 15, and 22, or whether pulsed serially at incubation days 5–7, 12–14, and 19–21, and chased the day following completion of the pulse. Regression analysis of decline in BrdU labeling of single pulse and serial chase generated a proliferative rate (LR50) of 6.7 days, and a coefficient of determination (R2) of 0.878. Weekly pulse with immediate chase generated a LU50 of 14.5 days, R2 value of 0.617.