Inhibition constants (IC50-values), relative inhibitory potency (RIP) of the glycomacromolecules 4, 9, 10 and glycomacromolecules functionalized liposomes L4, L9, L10.
| Glycoconjugate | No. of carbohydrate | C meas. ± SDa [μM] (Cmeas./C100% [%]) | IC50 ± SDb [μM] | RIPc | RIPligand/liposomed |
|---|---|---|---|---|---|
| Lac(1)-2, 4 | 1 | — | 123 ± 3 | 1.3 | — |
| Lac(1,3,5)-6, 9 | 3 | — | 38 ± 2 | 4.2 | — |
| Lac(1,2,3)-4, 10 | 3 | — | 37 ± 1 | 4.3 | — |
| Lac(1)-2, L4 | 1 | 143 ± 19 (94) | 12 ± 2 | 13 | 10 |
| Lac(1,3,5)-6, L9 | 3 | 127 ± 18 (94) | 1.0 ± 0.1 | 161 | 38 |
| Lac(1,2,3)-4, L10 | 3 | 107 ± 18 (66) | 0.3 ± 0.03 | 482 | 112 |
a
C meas. [μM]: concentration of the glycomacromolecules on the liposome surface determined with the lactose assay (percentage of glycooligomer relative to the theoretical concentration (Coligo/C100% [%])).
b
Determined by ELISA-inspired inhibition studies on ASF coated plates. Measurements were performed two times in triplicates.
c
Relative inhibitory potency (RIP) normalized to IC50 value of lactose (159 μM).
d
RIP of the liposomes compared to the corresponding single ligand in solution.