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. 2021 Sep 22;12(3):1363–1376. doi: 10.1016/j.apsb.2021.09.016

Figure 5.

Figure 5

[68Ga]DPA PET imaging of PD-L1 overexpressed U87MG tumor. (A) U87MG cell uptake of radioactivity when incubated with [68Ga]DPA (20 μCi/mL) or [68Ga]DPA (20 μCi/mL) + BMS202 (100 μmol/L) for different periods. (B) Competitive binding of [68Ga]DPA (20 μCi/mL) to U87MG cells co-incubated with different concentrations of BMS202 for 120 min at 37 °C. Ki value is shown in the panel. (C) PET-CT images of U87MG bearing BALB/c nude mice (left panel) and Bon-1 bearing BALB/c nude mice (right panel) treated with approximately 0.5 mCi of [68Ga]DPA. Mice were imaged at 30 min and 60 min after intravenous injection of [68Ga]DPA. Representative MIP (top panel) and transverse PET-CT images (bottom panel) are shown. The white dashed circles indicate the tumor positions. (D) and (E) Whole-section immunohistochemical staining of PD-L1 in U87MG and Bon-1 tumors. (F) Enlarged view of the selected areas in (D) and (E). Scale bar, 100 μm. (G) H&E staining of U87MG and Bon-1 tumor tissues. Scale bar, 100 μm. (H) Biodistribution of [68Ga]DPA in tissue from U87MG bearing BALB/c nude mice measured by gamma counter ex vivo after 5, 30, 60, and 120 min intravenous injection of the tracer. n = 4 mice per time point. (I) Ratios of tissue uptake of [68Ga]DPA. Values were calculated based on the ex vivo biodistribution data in (G). Data are mean ± SD, n = 4.