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. 2021 Sep 22;12(3):1198–1212. doi: 10.1016/j.apsb.2021.09.017

Figure 4.

Figure 4

LPS stimulation induces an elevated expression of NMMHC IIA and downregulation of VE-cadherin in pulmonary endothelium. (A‒D) NMMHC IIA, TLR4, p-Src (Tyr416), and VE-cadherin expression in pulmonary endothelium after LPS stimulation was assessed based on immunofluorescence with anti-CD31 (red), anti-NMMHC IIA (green), anti-TLR4 (green), anti-p-Src (green), and anti-VE-cadherin (green), and visualized using confocal microscopy. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. (E‒H) Fluorescence intensity of NMMHC IIA, TLR4, p-Src, and VE-cadherin in vessels was quantified by “Colocalization Analysis” using Image J. All data are expressed as mean ± SD, n = 3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. 0 h group.