Figure 8.

NMMHC IIA binds to TLR4 through its N-terminal and head domains. (A) Binding mode analysis of NMMHC IIA and BB loop of TLR4 TIR domain via molecular docking (blue represents NMMHC IIA and red represents the binding probability of BB loop and NMMHC IIA). (B) Homology modeling of the complex of TLR4 TIR domain and NMMHC IIA. (C) Co-IP analysis was used to determine interactions between different domains of NMMHC IIA and TLR4. (D) BLI analysis was employed to detect the direct interaction between NMMHC IIA head domain and TLR4. (E, F) HEK293T cells were co-transfected with plasmids encoding Myc-tagged TLR4 and GFP-tagged Myh9 N-terminal or head domain mutants. Cell lysates were precipitated with anti-GFP antibody and immunoprecipitates detected via Western blot using anti-Myc and anti-GFP antibodies.