Figure 2.

Silica induces GSDMD-dependent pyroptosis in macrophages. (A) Cell morphology was visualized by phase contrast microscope (top) and SEM (bottom). The arrows indicate bubbling of pyroptotic cells. (B) Concentration of IL-1β and (C) activity of LDH in cell supernatant. (D) Cell viability was monitored by PI detection for 120 min. (E) Western blot of GSDMD^FL and GSDMD^NT. (F) The statistical analysis of band densities in E. The data are reported as the mean ± SE. All experiments were repeated independently for three times (n = 3 per group). ∗∗P < 0.01, ∗∗∗P < 0.001.