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. 2021 Oct 30;12(3):1390–1405. doi: 10.1016/j.apsb.2021.10.024

Figure 5.

Figure 5

Complex 1 induced cell apoptosis and inhibited cell migration through inhibition of c-Myc and Mcl-1 protein level. (A) MDA-MB-231 and 4T1 cells were treated with the indicated concentrations of 1 or 20 treated for 4 h. Cell lysates were collected and immunoblotted with the anti-apoptosis corresponding antibodies. (B–C) The effect of 1 on CSC (B) and EMT (C) biomarkers levels in MDA-MB-231 and 4T1 cells. DMSO or indicated concentrations of 1 or 20 were treated with MDA-MB-231 and 4T1 cells for 4 h. Then cells were collected and incubated with appropriate antibodies. (D) The anti-proliferative effect of 1 in MDA-MB-231 cells after transfection with c-Myc and Mcl-1. Cells were transfected with c-Myc, Mcl-1, or control plasmid for 48 h, then treated with DMSO or 1 for 48 h. Cell viability was detected by the MTT assay. (E) 1 inhibited the migration of TNBC cell lines. Indicated concentrations of 1 or 20 treated with MDA-MB-231 and 4T1 cells for 24 h and evaluated by transwell. (F–G) Quantitative analysis of migration cells. Data are presented as the mean ± SD. ∗P < 0.05, ∗∗P < 0.01 compared to DMSO group by the t-test.