Figure 6.
Complex 1 suppresses tumor growth, lung metastasis, and proliferation in a TNBC mouse model. Female BALB/c mice were subcutaneously inoculated with 1.0 × 106 4T1 cells, and after the tumor volume reached 60 mm3, injected intraperitoneally daily with 1 or 2 or 4 mg/kg of complex 1 or 0.5 mg/kg of cisplatin for 18 days. (A) Tumor volume of 4T1 tumor-bearing mice after control (vehicle), 1 (1, 2 or 4 mg/kg) and cisplatin (0.5 mg/kg) treatment (n = 6–8). (B) Body weight of tumor-bearing mice over the course of treatment. (C) Comparison of tumor weight after sacrifice. (D) The percentage of metastatic burden area. (E) Representative photograph images of lung metastatic tissues and H&E stained lung sections in vehicle control, complex 1, and cisplatin groups after 18 days. Scale bar = 200 μm (×40). (F) Six random PCNA-stained areas of a single tumor from each group are shown at ×40 magnification. (G) PCNA histological score is displayed. Six random areas of each tumor section are enrolled, and each dot represents the score of one area. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to vehicle by the t-test.