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. 2021 Nov 9;12(3):1305–1321. doi: 10.1016/j.apsb.2021.11.002

Figure 3.

Figure 3

Effects of RG108 on cisplatin-induced hearing loss in vivo. (A) Experimental workflow. (B) ABR thresholds at 8, 16, 24, 32 kHz were measured 14 days after injection of saline (Con), cisplatin (Cis), cisplatin plus 10 mg/kg RG108 (RG108-Cis), and 10 mg/kg RG108 only (RG108) (each group, n = 6 mice). (C) Immunostaining with myosin7a (red) and Alexa Fluor 488 phalloidin (green) in the apical, middle, and basal turns of cochleae from different groups. Scale bars = 20 μm. (D) The illustration of HC counting (myosin7a, red) (n = 9–13 cochleae). (E) Immunostaining with myosin7a (red) and Sox2 (white) in the inner ear sections of mice from different group. Scale bars = 20 μm. (F) Representative images of Tuj-1 (green) staining of SGNs at apical, middle, and basal turns of the cochlea from different groups. Scale bar = 20 μm. (G) The illustration of SGN counting (Tuj-1, green) (n = 10 cochleae). (H) Immunostaining with Tuj-1 (green) and TUNEL (red) in the middle turns of SGNs from different groups. Scale bars = 20 μm. The data represent mean ± SEM; ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 vs. Con; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. Cis. IHCs, inner hair cells; OHCs, outer hair cells.