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. 2021 Nov 9;12(3):1305–1321. doi: 10.1016/j.apsb.2021.11.002

Figure 5.

Figure 5

Effects of RG108 on cisplatin-induced apoptosis in HEI-OC1 cells. (A) Cell viability was determined by the CCK-8 assay. (B) and (C) Flow cytometry analysis of RG108 on cell apoptosis induced by cisplatin. Apoptotic cells were detected by Annexin V-FITC/PI kit. (D) Representative images of APAF1 staining in HEI-OC1 cells. (E) Representative images of caspase-3/7 staining in HEI-OC1 cells. (F) Flow cytometry analysis of caspase-3/7 in HEI-OC1 cells. (G) Quantitative changes in the caspase-3/7 were analyzed by flow cytometry data. (H) Representative images of TUNEL staining in HEI-OC1 cells. (I) Quantification of TUNEL positive cells. TUNEL staining was quantified by counting the TUNEL staining-positive cells and then normalizing to the total cell number in each image. Four different fields were selected from a coverslip, and three independent experiments were performed. Scale bars = 10 μm. Data are shown as mean ± SEM. ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 vs. Con; #P < 0.05, ####P < 0.0001 vs. Cis.