Figure 3. Microwave radiation inhibited miR-30a expression both in rat hippocampi and neuron-like cells.
(A) Differentially expressed miRNAs in the rat hippocampi screened by miRNA chip. (B,C) The expression of miR-30a and U6 in rat hippocampi 7 days, 14 days and 1 month after radiation and PC12 cell-derived neuron-like cells 6, 12 and 24 h after radiation, respectively, which were detected by real-time RT-PCR. (D) Representative images of miR-30a and U6 in rat hippocampi 7 and 14 days after radiation, detected by ISH (scale bar = 30 μm). (E) The quantitative analysis result of the ratio of miR-30a to U6 presented in (D). The data are presented as mean ± SEM. One-way ANOVA with repeated measures was used to analyze the time course, and Student’s t test was used to compare the difference between the two groups: SH and MW (B,C,E). *, P<0.05; **, P<0.01; ***, P<0.001 vs the control.