Table 11.
Reported optical probes for GSTs.
| Enzyme | No. | Substrate | Metabolite | Km (μmol/L) | Vmax or Kcat | λex/λem (nm) | Ref. |
|---|---|---|---|---|---|---|---|
| GSTs | GSTs-F1 |  |
 |
1107 × 104 L/mol·s for hGSTA1 | N.A. | 492/516 | 127 |
| GSTs | GSTs-F2 |  |
 |
21.34 | Kcat = 389 S‒1 | 730/810 nm | 128 |
| GSTs | GSTs-F3 |  |
 |
84 | Vmax = 3.84 μmol/min/L | 420/550 | 135 |
| GSTs | GSTs-F4 |  |
 |
0.31 for hGSTM1-1 | N.A. | 505/525 | 132 |
| GSTs | GSTs-F5 |  |
 |
N.A. | N.A. | 540/620 | 133 |
| GSTs | GSTs-F6 |  |
 |
3.2 ± 0.3 for GSTA1-1; 4.9 ± 0.8 for GSTM2-2; 4.1 ± 0.4 for GSTP1-1 | Kcat = 11.36 ± 0.8 S‒1 for GSTA1-1; 2.09 ± 0.19 for GSTM2-2; 0.46 ± 0.03 for GSTP1-1 | 490/522 | 134 |
| GSTs | GSTs-B1 |  |
 |
39.3 ± 3.1 | N.A. | Biolumincence | 136 |
| GSTs | GSTs-B2 |  |
 |
2.8 ± 0.53 for GSTM1-1; 3.7 ± 0.59 for GSTP1-1 | Kcat = 0.0006 S‒1 for GSTM1-1; Kcat = 0.0009 S‒1 for GSTP1-1 | Biolumincence | 137 |
| MGST1 | MGST1-F1 |  |
 |
1.7 ± 0.5 | NA | 499/522 | 194 |
| GSTP1 | GSTP1-F1 |  |
 |
N.A. | N.A. | 493/510 | 130 |
| GSTP1 | GSTP1-F2 |  |
 |
13.3 ± 2.8 | Kcat = 0.01±0.0017 S-1 | 389/503 | 131 |
| GSTP1 | GSTP1-F3 |  |
 |
0.39 | Vmax=0.0357 μmol/min/L | 670/695 | 195 |
| GSTP1 | GSTP1-F4 |  |
 |
6.4 | Kcat= 0.6 S‒1 | 490/510 | 196 |
| GSTA1-1 | GSTP1A1-F1 |  |
 |
N.A. | N.A. | 495/553 | 197 |
| GSTM1 | GSTM1-F1 |  |
 |
1.55 for HLS9 | N.A. | 530/588 790/588 |
129 |
| GST | GST-F1 |  |
 |
489.35 | Vmax=7.08 μmol/min/L | 490/510 | 198 |