TABLE 3.

Differences in the PBP 5 from E. faecium D344, H80721, and BM4107^a

Positionb	Amino acid
	BM4107	D344	H80721
Membrane anchor
24	V	A	A
25	T	A	A
27	S	G	G
34	R	Q	Q
39	S	T	T
N-terminal extension
66	G	E	E
68	A	T	T
85	E	D	D
94	A	T	A
100	E	Q	Q
144	K	Q	Q
N-terminal domain
172	T	A	A
177	L	I	I
204	D	G	G
216	A	S	S
324	T	A	A
C-terminal PBP domain
404	N	N	D
466′	None	None	S
485	Mc	T	A
496	N	K	K
499	A	T	T
525	E	D	D
601	N	Y	N
626	K	E	K
629	E	V	V
654	D	N	N
663	T	A	T
667	P	S	S

^aDeduced amino acid sequence of pbp5 genes from E. faecium H80721 (GenBank accession no. X84862), D344 (GenBank accession no. AF362954), and BM4107 (GenBank accession no. AF364092). 

^bNumbering of PBP 5 from E. faecium H80721. Positions were assigned to the different domains of the PBP as previously described (21). 

^cA Met-to-Ala substitution was introduced by site-directed mutagenesis at this position (see pSF4 in Table 1).