TABLE 4.

Structures of muropeptides from E. faecium

Peak no.b	Peptidoglycan structurec	Amt (% total)a
		D344	D344 + CR064d	D344S
Monomers		41.7	42.0	33.7
1	ds-di	0.9	0.7	0.5
2	ds-tri	6.6	5.8	4.7
3	ds-tetra	2.5	3.2	3.4
4	ds-Asp-tri	4.1	2.8	1.5
5	ds-Asn-tri	16.3	16.2	13.0
6	ds-Asp-tetra	2.7	3.4	1.5
7	ds-Asn-tetra	3.2	5.5	4.4
8	ds-Asn-penta	0.6	2.4	1.8
9	ds(Ac)-Asp-tri	1.2	0.5	0.4
10	ds(Ac)-Asn-tri	3.6	1.5	2.5
Dimers and  trimers		58.3	58.0	66.3
11	Bis-ds-tetra-Asn-tri	4.5	6.3	3.2
12	Bis-ds-Asp-tetra-Asn-trie	4.3	3.6	5.2
13	Bis-ds-Asn-tetra-Asn-tri	17.1	18.2	19.6
14	Bis-ds-Asp-tetra-Asn-tetrae	1.4	1.6	1.3
15	Bis-ds-(Ac)-tetra-Asn-tri	1.1	1.7	1.1
16	Bis-ds-Asn-tetra-Asn-tetra	4.2	7.0	8.6
17	Bis-ds-(Ac)-Asp-tetra-Asn-trie	1.8	2.1	1.4
18	Bis-ds-Asp-tetra-Asn-tetrae	1.7	1.9	3.5
19	Bis-ds-(Ac)-Asn-tetra-Asn-tri	6.4	4.0	6.7
20	Ter-ds-Asn-tetra-Asn-tetra-Asn-tri	6.2	5.6	6.5
21	Bis-ds-(AcX2)-Asp-tetra-Asn-trie	2.7	2.5	4.0
22	Bis-ds-(AcX2)-Asn-tetra-Asn-tri	6.9	3.5	5.2

^aThe values are presented as a percentage of the sum of all peaks presented in the table and are the means of two experiments. 

^bMuropeptide peak designations as in reference 14. 

^cAbbreviations: ds, disaccharide (N-acetylglucosamine-β-1.4-N-acetylmuramic acid); Bis, dimeric form; Ter, trimeric form; di, dipeptide (l-alanyl-d-isoglutamine); tri, tripeptide (l-alanyl-d-isoglutamyl-l-lysine); tetra, tetrapeptide (l-alanyl-d-isoglutamyl-l-lysyl-d-alanine); penta, pentapeptide (l-alanyl-d-isoglutamyl-l-lysyl-d-alanyl-d-alanine); Asn, d-asparagine; Asp, d-aspartate; Ac, O-acetylation located on N-acetylmuramic acid; ACx2, O-acetylation on both N-acetylmuramic acids of the dimer. 

^dD344 was grown in presence of 64 μg of ceftriaxone per ml (CRO64). 

^eAssignment of Asp and Asn residues to either stem peptide is arbitrary.